Cysteine biosynthesis in plants: isolation and functional identification of a cDNA encoding a serine acetyltransferase from Arabidopsis thaliana

FEBS Letters

Hell, Rüdiger¹  Bork, Christiane¹  Bogdanova, Natascha¹ 

[1] Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum 44780 Bochum, Germany

关键词: Cysteine synthesis;    Serine acetyltransferase;    Sulfate assimilation;    Complementation;    Arabidopsis thaliana;    SATase;    serine acetyltransferase;    OAS-TL;    O-acetylserine (thiol) lyase;    EST;    expressed sequencing tag;    1PTG;    isopropyl-β-d-thiogalactoside;   

DOI  :  10.1016/0014-5793(94)01392-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding for serine acetyltransferase which catalyzes the committing step of cysteine biosynthesis has been cloned from Arabidopsis thaliana. The plant protein has a predicted molecular weight of 32.8 kDa and shows up to 43% of amino acid homology to bacterial serine acetyltransferases. It complements a serine acetyltransferase negative E. coli mutant and can be enzymatically determined in the heterologous host. The corresponding mRNA is predominantely expressed in light exposed tissue and represents one of at least two related genes.

【 授权许可】

Unknown