The NssBF element, a sequence of the Drosophila melanogaster retrotransposon 1731 potentially implicated in transcriptional repression and replication

FEBS Letters

Fourcade-Peronnet, Frédérique¹  Lacoste, Jérôme¹ 

[1] UA CNRS 1135, Groupe de Génétique Cellulaire et Moléculaire, Université P. et M. Curie, 7, quai St-Bernard, 75005 Paris, France

关键词: Retrotransposon 1731;    Drosophila melanogaster;    Single-stranded DNA binding protein;    Repression;    Replication;   

DOI  :  10.1016/0014-5793(94)01373-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nuclear single-stranded DNA binding factor (NssBF) has been characterized as a nuclear protein that binds to a 26 nucleotides sequence in the long terminal repeat (LTR) of the Drosophila melanogaster 1731 retrotransposon. This sequence, called NssBF element, was analysed by gel retardation experiments using wild-type and mutated oligonucleotides. In vitro transcription experiments were performed and suggest that NssBF element binding protein(s) represses transcription through the 1731 promoter. Furthermore, computer assisted sequence comparisons put forward a possible role of this element and/or its associated DNA binding proteins in replication.

【 授权许可】

Unknown