Angiography contrast agents interact with serine proteinases The molecular structure of the model system elastase/iohexol

FEBS Letters

Corot, Claire¹  Gaudey, Françoise²  Avenel, Danièle²  Ohanessian, Jacqueline²  Neuman, Alain²  Prangé, Thierry²  Meyer, Dominique¹ 

[1] Guerbet-GCA, Service Immuno-Hemato-Biochimie, Z.I. de l' Islon, 38670 Chasse-Sur-Rhône, France;Chimie Structurale Biomoléculaire (URA 1430 CNRS) 74, rue Marcel Cachin, 93012 Bobigny Cedex, France

关键词: Iohexol (tri iodobenzoic);    Elastase;    X-ray Diffraction;    Angiography;    Proteinase;    Inhibition;   

DOI  :  10.1016/0014-5793(94)01356-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pancreatic elastase was co-crystallised with iohexol, a tri-iodo benzenic contrast agent used in angiography analyses. The X-ray analysis of the complex reveals the presence of three molecules of iohexol associated with the proteinase with low occupancy factors. Two iohexol molecules are located in and near the active site of the enzyme and provide a model for explaining the inhibition of the hemostatic system, one of the major and inconvenient side effect associated with these chemicals.

【 授权许可】

Unknown