Inducible nitric oxide synthase in human lymphomononuclear cells activated by synthetic peptides derived from extracellular matrix proteins

FEBS Letters

Montuenga, L.¹  Calonge, M.²  López-Moratalla, N.²  Pérez-Mediavilla, L.A.²  López-Zabalza, M.J.²  Santiago, E.² 

[1] Department of Cytology and Histology, University of Navarra, Pamplona, Spain;Department of Biochemistry, University of Navarra, Pamplona, Spain

关键词: Nitric oxide synthase (inducible);    NO release;    Human lymphomononuclear cell;    Immunocytochemistry;    Extracellular matrix protein;    iNOS;    inducible nitric oxide synthase;    SpA;    Staphilococcus aureus protein A;    LPS;    lipopolysaccharide;    NMMA;    N G-monomethyl-l-arginine;   

DOI  :  10.1016/0014-5793(94)01322-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthetic peptides with sequences present in extracellular matrix proteins are capable of causing the expression of the inducible form of nitric oxide synthase (iNOS), detected by immunocytochemistry, and the release of NO by human lymphomononuclear cells incubated in their presence. Active peptides are 15-mers containing a characteristic 2-6-11 motif in which the amino acid residue at position 2 is Leu, Ile, Val, Gly, Ala or Lys; the residue at position 6 is always Pro; and residue 11 is Glu or Asp. The induction of iNOS in human monocytes and macrophages could be involved in the cytotoxicity against tumor cell lines also elicited by these peptides.

【 授权许可】

Unknown