Cloning and characterization of the Drosophila melanogaster CDK5 homolog

FEBS Letters

Hellmich, Mark R.¹  Hampton, Lori L.¹  Kennison, James A.²  Battey, James F.¹ 

[1] Laboratory of Biological Chemistry, Developmental Therapeutics Program, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Building 37, Room 5D02, 9000 Rockville Pike, Bethesda, MD 20892, USA;Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA

关键词: CDK5;    cDNA;    Cyclin-dependent kinase;    Development;    Drosophila;   

DOI  :  10.1016/0014-5793(94)01298-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The D. melanogaster homolog of mammalian CDK5 has been cloned and its chromosomal location determined. The gene for Cdk5 consists of 4 exons separated by 3 short introns ranging in size from 61–160 bp. Northern blot analysis revealed a single mRNA of approximately 1.6 kb that is expressed at highest levels in the adult fly. The putative amino acid sequence for Drosophila Cdk5 predicts a protein with a mass of approximately 32 kDa that is 77% identical to its mammalian counter-parts. Drosophila Cdk5 gene is located in polytene chromosomal region 52BC of the right arm of chromosome 2. This study provides the framework for a molecular genetic analysis of CDK5 function.

【 授权许可】

Unknown