The reversion of highly tumorigenic cell lines to non‐tumorigenic phenotype is associated with c‐jun down‐expression

FEBS Letters

Lavrovsky, Vadim¹  Yefremov, Yaroslav²  Lavrovsky, Yan³ 

[1]Institute of Cytology and Genetics (V.L.), Novosibirsk 630090, Russian Federation

[2]Institute of Bioorganic Chemistry, Novosibirsk 630090, Russian Federation

[3]The Rockefeller University, 1230 York Ave., New York, NY 10021, USA

关键词: Revertant;    Transformation;    Tumorigenicity;    Oncogene;    AP-1;    CAT;    chloramphenicol acetyltransferase;    FCS;    fetal calf serum;    AP-1;    activator protein 1;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/0014-5793(94)01266-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using model spontaneously reverting cell lines, c-jun, junB, junD and c-fos oncogene expression was investigated. c-jun, but not junB, junD or c-fos, was overexpressed in highly tumorigenic clones. The reversion of cells to the non-tumorigenic phenotype resulted in a dramatic decrease in c-jun expression. CAT assays revealed that c-jun overexpression in tumorigenic cells was associated with higher transcription activity. No correlation between c-jun oncogene expression and AP-1 transcription factor activity in tumorigenic and non-tumorigenic clones was found.

【 授权许可】

Unknown