Singlet molecular oxygen production in the reaction of peroxynitrite with hydrogen peroxide

FEBS Letters

Di Mascio, Paolo²  Sies, Helmut¹  Briviba, Karlis¹  Medeiros, Marisa H.G.²  Bechara, Etelvino J.H.² 

[1] Institut für Physiologische Chemie I, Heinrich-Heine- Universität Düsseldorf, PO Box 101007, D-40001 Düsseldorf Germany;Instituto de Química, Departamento de Bioquímica, Universidade de São Paulo, CP 20780, CEP 01498-970, São Paulo, Brazil

关键词: Singlet molecular oxygen;    Peroxynitrite;    Nitric oxide;    Hydrogen peroxide;    Chemiluminescence;    Near-infrared emission;   

DOI  :  10.1016/0014-5793(94)01224-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peroxynitrite and hydrogen peroxide are mediators of cytotoxicity. This study shows that the peroxynitrite anion reacts with hydrogen peroxide to release oxygen accompanied by emission of chemiluminescence (CL). Direct characterization of this light emission attributes it to the transition of singlet molecular oxygen to the triplet ground state. Chemiluminescence was monitored: (i) by dimol light emission in the red spectral region (> 610 nm) using a red-sensitive photomultiplier; and (ii) by monomol light emission in the infrared (1270 nm) with a liquid nitrogen-cooled germanium diode. These properties of photoemission and the enhancing effect of deuterium oxide on CL intensity as well as the quenching effect of sodium azide are diagnostic of molecular oxygen in the excited singlet state. For comparison, singlet molecular oxygen arising from the thermolysis of the water-soluble endoperoxide of 3,3′-(1,4-naphthylidene)dipropionate or from the hypochlorite/H₂O₂ system was also monitored. These novel observations identify a potential singlet oxygen-dependent mechanism contributing to cytotoxicity mediated by peroxynitrite and hydrogen peroxide.

【 授权许可】

Unknown