Inhibition of growth and cysteine proteinase activity of Staphylococcus aureus V8 by phosphorylated cystatin α in skin cornified envelope

FEBS Letters

Tezuka, Tadashi¹  Katunuma, Nobuhiko²  Takahashi, Masae¹ 

[1] Department of Dermatology, Kinki University School of Medicine, 377-2, Ohno-Higashi, Osaka-Sayama, Osaka 589, Japan;Institute for Health Sciences, Tokushima Bunri University, Yamashiro, Tokushima, Tokushima 770, Japan

关键词: Phosphorylated cystatin α;    Cornified envelope;    Epidermal barrier function;    Staphylococcus aureus V8;    P-cystatin α;    phosphorylated cystatin α;    PoAb;    polyclonal antibody;    SAV8;    Staphylococcus aureus V8;   

DOI  :  10.1016/0014-5793(94)01196-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activity of a cysteine proteinase purified from Staphylococcus aureus V8 (SAV8) was inhibited by phosphorylated cystatin α (P-cystatin α) and by purified cornified envelope protein of newborn rat, a conjugated form of P-cystatin α. Immunohistochemical analysis demonstrated a marked decrease in P-cystatin α content in cornified envelope treated with sphingosine. The inhibition of papain activity by proteins from sphingosine-treated skin was much weaker than that exerted by proteins from the untreated skin. The suppression of SAV8 colony formation inoculated on the sphingosine-treated skin was examined. Colony formation on the sphingosine-treated skin was enhanced compared to that on normal skin. These findings suggest that P-cystatin α in the cornified envelope may have a bacteriostatic barrier function against bacterial infection, such as that with SAV8.

【 授权许可】

Unknown