Pyridine dinucleotide biosynthesis in archaebacteria: Presence of NMN adenylyltransferase in Sulfolobus solfataricus

FEBS Letters

Ruggieri, Silverio¹  Emanuelli, Monica¹  Amici, Adolfo¹  Raffaelli, Nadia¹  Magni, Giulio¹ 

[1] Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università di Ancona, Via Ranieri, 60131 Ancona, Italy

关键词: Archea;    Thermophile;    NAD;    NMN adenylyltransferase;    NaMN;    nicotinic acid mononucleotide;    NaAD;    nicotonic acid adenine dinucleotide;    DTT;    dithiothreitol;   

DOI  :  10.1016/0014-5793(94)01195-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The enzyme NMN adenylyltransferase, leading to NAD synthesis, has been observed for the first time in soluble extracts from the extreme acidothermophilic archaeon Sulfolobus solfataricus. Comparison of its molecular and kinetic properties with those of the enzyme isolated from prokaryotes and eukaryotes revealed significant differences, knowledge of which may contribute to the understanding of metabolic evolutionary mechanisms. The thermophilic enzyme shows a molecular mass of about 66,000 and an isoelectric point of 5.4. The K _m values for ATP, NMN and nicotinic acid mononucleotide are 0.08 μM, 1.4 μM and 17 μM, respectively. The enzyme shows a remarkable degree of thermophilicity, with an activation energy of 95 kJ/mol.

【 授权许可】

Unknown