Inhibition of the respiratory burst in mouse macrophages by ultra‐low doses of an opioid peptide is consistent with a possible adaptation mechanism

FEBS Letters

Borodulina, O.I.¹  Koshkin, A.A.¹  Efanov, A.M.¹  Sazanov, L.A.¹  Varfolomeev, S.D.¹  Zaitsev, S.V.¹ 

[1] Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation

关键词: Ultra-low dose;    Kinetic mechanism;    Opioid peptide;    Macrophage;    Respiratory burst;    DAMEA;    [d-Ala2]methionine enkephalinamide;    PMA;    phorbol 12-myristate 13 acetate;    ROS;    reactive oxygen species;   

DOI  :  10.1016/0014-5793(94)01109-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The respiratory burst induced by phorbol myristate acetate in mouse macrophages was inhibited by ultra-low doses (10⁻¹⁵ −10⁻¹³ M) of an opioid peptide [d-Ala²] methionine enkephalinamide. The effect disappeared at concentrations above and below this range. The inhibition approached 50% and was statistically significant (P < 0.001). Increasing the time of the opioid incubation with cells brought about a shift in the maximal effect to lower concentrations of the opioid (from 10⁻¹³ to 5 · 10⁻¹⁵ M) and led to a decrease in the value of the effect, fully in accord with the previously proposed adaptation mechanism of the action of ultra-low doses.

【 授权许可】

Unknown