Hybrid proteins between Pseudomonas exotoxin A and poliovirus protease 2Apro

FEBS Letters

Feduchi, Elena¹  Carrasco, Luis¹  Novoa, Isabel¹ 

[1] Centro de Biología Molecular, UAM-CSIC, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain

关键词: Poliovirus 2Apro;    Initiation factor;    Pseudomonas exotoxin A;    Viral protease;   

DOI  :  10.1016/0014-5793(94)01157-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two hybrid proteins between Pseudomonas aeruginosa exotoxin A (PE) and poliovirus protease 2A^pro have been generated. One hybrid protein contains the poliovirus 2A^pro sequence replacing the region of PE corresponding to amino acids 413–607. The other hybrid contains in addition the transforming growth factor sequence. The two hybrid proteins were efficiently synthesized in E. coli cells using the inducible pET vectors. Both hybrid toxins cleaved p220 (eIF-4γ) when the recombinant plasmids were transfected in COS cells infected with recombinant vaccinia virus bearing the T7 RNA polymerase gene.

【 授权许可】

Unknown