pH‐independent inhibition of restriction endonuclease cleavage via triple helix formation by oligonucleotides containing 8‐oxo‐2′‐deoxyadenosine

FEBS Letters

Yamakawa, Hidefumi¹  Takai, Kazuyuki¹  Takaku, Hiroshi¹  Tsukahara, Satoru¹  Wang, Qing¹ 

[1] Department of Industrial Chemistry, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba 275, Japan

关键词: Triplex formation;    Base modification;    T m value;    Conformation;    Native polyacrylamide gel electrophoresis;    Inhibition of restriction enzyme;   

DOI  :  10.1016/0014-5793(94)01139-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ability of homopyrimidine oligonucleotides containing 8-oxo-2′-deoxyadenosine to form stable, triple helical structures with the sequence containing the recognition site for the class II-S restriction enzyme, Ksp632-I, was studied as a function of pH. The 8-oxo-2′-deoxyadenosine-substituted oligomers were shown to inhibit enzymatic cleavage and to bind within the physiological pH range in a pH-independent fashion without compromising specificity.

【 授权许可】

Unknown