Very low density lipoprotein receptor from mammary gland and mammary epithelial cell lines binds and mediates endocytosis of M r, 40,000 receptor associated protein

FEBS Letters

Rasmussen, Lone K.²  Andreasen, Peter A.¹  Christensen, Anni¹  Etzerodt, Michael³  Heegaard, Christian W.¹  Ellgaard, Lars³  Kjøller, Lars¹  Wiborg Simonsen, Anna Carina¹ 

[1] Department of Molecular Biology, 130 C.F. Møller's Allé, University of Aarhus, DK-8000 Aarhus, Denmark;Protein Chemistry Laboratory, 10 Gustav Wieds Vej, University of Aarhus, DK-8000 Aarhus, Denmark;Laboratory of Gene Expression, 10 Gustav Wieds Vej, University of Aarhus, DK-8000 Aarhus, Denmark

关键词: Endocytosis;    Cell membrane;    Ligand blotting;    MCF-7 cell;    α2M*;    α2-macroglobulin-methyl amine;    α2MR/LRP;    α2-macroglobulin receptor/low density lipoprotein receptor-related protein;    CHAPS;    3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propanesulfonate;    gp330;    glycoprotein 330;    LDL-R;    low density lipoprotein receptor;    RAP;    M r 40;    000 receptor associated protein;    PBS;    phosphate-buffered saline;    PMSF;    phenyl-methyl-sulfonyl flouride;    PVDF;    polyvinylidine diflouride;    SDS-PAGE;    sodium dodecyl sulphate polyacryl amide gel electrophoresis;    TCA;    trichloroacetic acid;    VLDL-R;    very low density lipoprotein receptor;   

DOI  :  10.1016/0014-5793(94)01138-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We here report that the M _r 40,000 receptor associated protein (RAP), previously found to bind to α₂-macroglobulin receptor/low density lipoprotein receptor related protein (α₂MR/LRP) and glycoprotein 330 (gp330), binds to an M _r, 105,000 membrane protein from bovine mammary gland, human mamma tumors and mammary epithelial cell lines. We have purified this protein from bovine and human sources. N-terminal amino acid sequencing and immunoblotting analyses showed that the protein was identical or closely related to very low density lipoprotein receptor (VLDL-R). Experiments with the human mamma carcinoma cell line MCF-7 showed that this receptor was able to mediate an efficient endocytosis of RAP. These novel findings strongly suggest that RAP functions as a modulator of ligand binding to VLDL-R, similarly to α₂MR/LRP and gp330.

【 授权许可】

Unknown