The three dimensional structure of rat cytokine CINC/Gro in solution by homonuclear 3D NMR

FEBS Letters

Hanzawa, Hiroyuki²  Haruyama, Hideyuki²  Tsurufuji, Susumu¹  Watanabe, Kazuyoshi¹ 

[1]Institute of Cytosignal Research, Inc., 2-58, Hiromachi 1-Chome, Shinagawaku, Tokyo 140, Japan

[2]Analytical and Metabolic Research Laboratories, Sankyo Co. Ltd., 2-58, Hiromachi 1-Chome, Shinagawaku Tokyo 140, Japan

关键词: Homonuclear 3D NMR;    IL-8 family;    Three dimensional structure;    DANTE;    delays alternating with nutation for tailored excitation;    DQF-COSY;    double quantum filtered corellated spectorscopy;    FID;    free induction decay;    HOHAHA;    homonuclear Hartman-Hahn spectroscopy;    MLEV;    Malcom Levitt;    NMR;    nuclear magnetic resonance;    NOESY;    nuclear Overhauser enhancement spectroscopy;   

DOI  :  10.1016/0014-5793(94)01081-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The solution conformation of rat cytokine-induced neutrophil chemoattractant (CINC/Gro), a small protein consisting of 72 amino acid residues with proinflammatory activities, and a member of the interleukin 8 family corresponding to a counterpart of human Gro, was investigated with homonuclear 2D and 3D NMR spectroscopy. At each phase of the structural analysis, the homonuclear 3D NOESY-HOHAHA and HOHAHA-NOESY spectra afforded valuable data, removing ambiguities intractable by conventional 2D NMR techniques. CINC/Gro exists as a dimer in solution and contains a triple stranded anti-parallel β-sheet and C-terminal α-helix in the monomer structure, as observed in human IL-8, but non-trivial differences are also observed.

【 授权许可】

Unknown