Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity

FEBS Letters

Hedley, P.E.¹  Waugh, R.¹  Machray, Gordon C.¹  Davies, H.V²  Burch, L.³ 

[1]Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK

[2]Cellular and Evironmental Physiology Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK

[3]Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, UK

关键词: Sucrose cleavage;    Potato;    Solanum tuberosum;    Ferredoxin;   

DOI  :  10.1016/0014-5793(94)01105-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cloning of a 1332 bp cDNA from a potato (Solanum tuberosumL.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.

【 授权许可】

Unknown