Processing and secretion of rat α1‐microglobulin‐bikunin expressed in eukaryotic cell lines

FEBS Letters

Åkerström, Bo¹  Cedervall, Tommy¹  Bratt, Tomas¹ 

[1] Section of Molecular Signaling, Department of Medical and Physiological Chemistry, Lund University, Box 94, S-221 00 Lund Sweden

关键词: Furin;    Precursor;    COS cell;    CHO cell;    RPE.40 cell;    Post-translational modification;    FCS;    foetal calf serum;    CHO;    Chinese hamster ovary;    RPE;    resistance to Pseudomonas exotoxin A;    EMEM;    Earle's minimum essential media;   

DOI  :  10.1016/0014-5793(94)01087-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The precursor protein α₁-microglobulin-bikunin was cleaved to the same degree whether expressed in CHO cells or in mutated CHO cells, RPE.40 cells, suggested to lack a functional form of the intracellular protease furin. Thus, α₁-microglobulin-bikunin probably is not cleaved in vivo by furin. However, simultaneous overexpression of the precursor and furin in COS, CHO and RPE.40 cells increased the cleavage, suggesting that compartmentalisation and concentrations of protease and precursor are important for the cleavage, besides the in vitro specificity. Expression of α₁-microglobulin and bikunin alone gave different protein patterns on SDS-PAGE as compared to expression of the precursor and subsequent cleavage, suggesting that the precursor protein is important for the post-translational handling of α₁-microglobulin and bikunin.

【 授权许可】

Unknown