Expression of GATA‐binding transcription factors in rat hepatocytes

FEBS Letters

Noda, Chiseko¹  Kobune, Yoshiko¹  Ichihara, Akira¹  Matsuda, Koichi¹ 

[1] Institute for Enzyme Research, University of Tokushima, Kuramoto-cho, Tokushima 770, Japan

关键词: DNA-binding protein;    GATA-family;    GATA-4;    Rat hepatocyte;   

DOI  :  10.1016/0014-5793(94)01062-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recently, we demonstrated that a DNA-binding protein(s) is involved in transcriptional repression of the rat serine dehydratase gene in fetal liver. Here, we report that a GAT(A/T) motif is the target sequence for the DNA-binding protein. By screening a fetal liver cDNA library, we isolated a rat homolog of GATA-1. Rat GATA-1 expressed as a GST-fusion protein in E. coli bound to the GAT(A/T) motif in the serine dehydratase gene. Northern analysis show that GATA-1 and GATA-4 mRNAs are expressed in fetal hepatocytes.

【 授权许可】

Unknown