Cloning and expression of mRNA encoding prepro‐gonad‐inhibiting hormone (GIH) in the lobster Homarus americanus

FEBS Letters

Martens, Gerard J.M.¹  Van Herp, François¹  Sleutels, Frank J.G.T.¹  de Kleijn, Dominique P.V.¹ 

[1]Department of Animal Physiology, Faculty of Sciences, University of Nijmegen, Toernooiveld, 6525 ED Nijmegen, The Netherlands

关键词: Gonad-inhibiting hormone;    Neurohormone;    cDNA sequence;    Lobster;    Medulla terminalis ganglionic X-organ;   

DOI  :  10.1016/0014-5793(94)01055-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gonad-inhibiting hormone (GIH) is produced in the eyestalk X-organ sinus gland complex of male and female lobsters, and plays a prominent role in the regulation of reproduction, e.g. inhibition of vitellogenesis in female animals. To study this neurohormone at the mRNA level, we cloned and sequenced a cDNA which encodes GIH in the lobster Homarus americanus. The structure of preproGIH consists of a signal peptide and the GIH peptide itself. A comparative analysis revealed that lobster GIH, together with crab molt-inhibiting hormone, belongs to a separate group of the crustacean hyperglycemic hormone (CHH) peptide family which seems to be unique for crustaceans. Expression studies showed that GIH mRNA is expressed in the eyestalk, indicating that the neuroendocrine center in this optic structure is the only source of GIH. As this center modulates the other (neuro)endocrine organs in crustaceans, it is postulated that GIH regulates production and release of hormones involved in reproduction/molting processes.

【 授权许可】

Unknown