p53 mutant His175 identified in a newly established fallopian tube carcinoma cell line secreting interleukin 6

FEBS Letters

Rosenthal, Heike E.¹  Kreienberg, Rolf¹  Möbus, Volker J.¹  Tong, Xiao W.¹  Runnebaum, Ingo B.¹  Kieback, Dirk G.¹ 

[1] Molecular Biology Laboratory, Department of Obstetrics and Gynecology, University of Ulm, Prittwitzstrasse 43, 89075 Ulm, Germany

关键词: Fallopian tube carcinoma;    Tumor suppressor gene;    p53 mutation;    Interleukin 6;    Proliferation;   

DOI  :  10.1016/0014-5793(94)00953-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fallopian tube carcinoma is a lethal gynecologic malignancy. Etiologic factors are unknown. No experimental data on molecular alterations exist so far. For an in vitro model, we established the permanent human tubal carcinoma cell line FT-MZ-1. The median doubling time was 14 days with 24.2% in S phase. A point missense mutation of the p53 tumor suppressor gene resulting in the His¹⁷⁵ mutant was identified. Aberrant p53 protein accumulated in nucleus and cytoplasm. FT-MZ-1 substantially secreted interleukin 6 (Il-6) coinciding with the inactivation of p53 as a transrepressor on the Il-6 gene promoter.

【 授权许可】

Unknown