Molecular cloning of rat kynurenine aminotransferase: Identity with glutamine transaminase K

FEBS Letters

Benatti, Luca³  Cozzi, Liviana³  Okuno, Etsuo²  Breton, Jerome³  Speciale, Carmela¹  Schwarcz, Robert²  Mosca, Monica³ 

[1] B.U. Therapeutics, Pharmacia Research Center, Nerviano, Italy;Maryland Psychiatric Research Center, University of Maryland School of Medicine, Baltimore, MD, USA;BioScience Center, Nerviano, Italy

关键词: Kynurenine aminotransferase;    Glutamine transaminase K;    Cysteine conjugate β-1yase;    Excitatory amino acid;    Neuroprotection;    Kynurenic acid;   

DOI  :  10.1016/0014-5793(94)01003-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The enzyme kynurenine aminotransferase (KAT) catalyses the conversion of l-kynurenine to kynurenic acid. A combination of polymerase chain reaction techniques and hybridization screening was used to isolate a cDNA clone encompassing the entire coding region of KAT from rat kidney. Identification of the cDNA as coding for KAT was based both on the comparison of amino acid sequences obtained from purified rat KAT and on the expression of KAT activity in COS-1 cells transfected with the cDNA. RNA blot analysis indicated that KAT mRNA is widely expressed in rat tissues. Cultured cells transfected with the cDNA for KAT also showed glutamine transaminase K activity. Based mainly on sequence data, these results demonstrate that rat kidney KAT is identical with glutamine transaminase K.

【 授权许可】

Unknown