A membrane‐bound HIPIP type center in the thermohalophile Rhodothermus marinus

FEBS Letters

Nunes, Olga C.¹  Antunes, Aida M.²  da Costa, Milton S.¹  Teixeira, Miguel²  Pereira, Manuela M.² 

[1] Centro de Biologia Celular, Departamento de Zoologia, Universidade de Coimbra, 3049 Coimbra Codex, Portugal;Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande, APT 127, 2780 Oeiras, Portugal

关键词: HIPIP;    Halophile;    Thermophile;    EPR;    Iron-sulfur;   

DOI  :  10.1016/0014-5793(94)00985-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A HIPIP-type center was discovered in intact membranes of the thermohalophilic aerobe Rhodothermus marinus. In both the membrane-bound state and after detergent solubilization and partial purification, this center exhibits an almost axial EPR spectrum, with g-values at 2.13 and 2.03, similar to those of soluble HIPIP proteins isolated from purple bacteria. It has a high reduction potential, of 260 mV at pH 7.5. Rhodothermus HIPIP is involved in the main membrane-bound electron-transfer pathway, being reduced by NADH or succinate only in the presence of cyanide. The possible physiological function of this novel HIPIP-type center is discussed.

【 授权许可】

Unknown