Neomycin: A novel potent blocker of communication between T‐tubule and sarcoplasmic reticulum

FEBS Letters

Antoniu, Bozena¹  El-Hayek, Roque¹  Ikemoto, Noriaki¹  Yano, Masafumi¹ 

[1] Boston Biomedical Research Institute, 20 Staniford Street, Boston, MA 02114, USA

关键词: Excitation—contraction coupling;    Triad;    Specific inhibitor;    Neomycin;    MES;    2-(N)morpholino)ethanesulfonic acid;    PMSF;    phenylmethyl sufonyl fluoride;    RyR;    ryanodine receptor;    SR;    sarcoplasmic reticulum;    T-tubule;    transverse tubular system;   

DOI  :  10.1016/0014-5793(94)00869-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ca²⁺ release from the sarcoplasmic reticulum (SR) was induced in isolated triads by direct stimulation of the SR moiety by polylysine, or stimulation via chemical depolarization of the transverse tubule (T-tubule) moiety. Polylysine-induced release was blocked by neomycin with an IC₅₀ (the concentration for half-maximal inhibition) of O.3, μM. However, the IC₅₀ for neomycin block of depolarization-induced Ca²⁺ release sharply decreased in a voltage-dependent fashion, and it was 5.3 nM at a maximal extent of T-tubule depolarization. These results suggest that the high affinity binding of neomycin to the triad leads to the specific blocking of the signal transmission from T-tubule to SR.

【 授权许可】

Unknown