On the mobility behavior of a curved DNA fragment located in circular permutations

FEBS Letters

Madhusudan, K.¹  Majumdar, H.K.³  Kulkarni, B.D.²  Nair, T.Murlidharan²  Nagaraja, V.¹  Singh, Rajan³ 

[1] Center for Genetic Engineering, Indian Institute of Science, Bangalore-560 012, India;Chemical Engineering Division, National Chemical Laboratory, Pune-411 008, India;Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, Calcutta-700 032, India

关键词: Intrinsic curvature;    Cyclically permuted;    Cyclically located;   

DOI  :  10.1016/0014-5793(94)00880-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Experimental and theoretical investigations on the mobility behavior of a set of permuted fragments with a K-DNA insert is reported. The fragments with the permuted flanking sequences have the K-DNA insert located differentially with respect to the fragment ends. The fragment wherein the insert is located in the center showed maximum retardation as compared to fragments where the insert was at the end. The experimental analysis is also in accord with the theoretical investigation.

【 授权许可】

Unknown