Yeast GAL11 protein stimulates basal transcription in a gene‐specific manner by a mechanism distinct from that by DNA‐bound activators

FEBS Letters

Ohishi, Tomoko²  Sakurai, Hiroshi²  Amakasu, Hiroko¹  Fukasawa, Toshio² 

[1] Department of Microbiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan;Laboratory of Molecular Genetics, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

关键词: GAL111 protein;    Basal transcription;    Gene specificity;    Intermediary factor;    In vitro transcription;    Saccharomyces cerevisiae;   

DOI  :  10.1016/0014-5793(94)80098-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The GAL11 gene encodes an auxiliary transcription factor required for full expression of many, if not all, genes of the yeast Saccharomycescerevisiae. We have recently shown that GAL11/-encoded protein (GAL111p) enhances basal transcription from the CYC1 promoter in a cell-free transcription system [(1993) Proc. Natl. Acad. Sci. USA 90, 8382-8386]. Here we indicate that Ga111p stimulates basal transcription in a gene-specific manner in vitro. We further suggest that the mechanism underlying the transcriptional stimulation by Ga111p is distinct from that by DNA-bound activators, since Ga111p stimulated transcription in a reaction system where activators were unable to enhance transcription due to the lack of intermediary factors.

【 授权许可】

Unknown