Crystal structure of domain A of Thermus flavus 5S rRNA and the contribution of water molecules to its structure

FEBS Letters

Erdmann, V.A.¹  Lorenz, S.¹  Betzel, Ch.²  Schneider, Th.R.²  Wilson, K.S.²  Zhang, M.¹  Bald, R.¹  Fürste, J.P.¹ 

[1] Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany;EMBL c/o DESY, Notkestraße 85, 22603 Hamburg, Germany

关键词: rRNA;    Solid phase synthesis;    Synchrotron radiation;    Solvent structure;   

DOI  :  10.1016/0014-5793(94)00834-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This is the first high resolution crystal structure of an RNA molecule made by solid phase chemical synthesis and representing a natural RNA. The structure of the domain A of Thermus flavus ribosomal 5S RNA is refined to R = 18% at 2.4 Å including 159 solvent molecules. Most of the 2′-hydroxyl groups as well as the phosphate oxygens are involved either in specific hydrogen bonds in intermolecular contacts or to solvent molecules. The two U-G and G-U base-pairs are stabilized by H-bonds supplied via three water molecules to compensate for the lack of base-pair hydrogen bonds. The structure shows for the first time in detail the importance of highly ordered internal water in stablizing an RNA structure.

【 授权许可】

Unknown