Direct 1H NMR evidence for conversion of β‐d‐cellobiose to cellobionolactone by cellobiose dehydrogenase from Phanerochaete chrysosporium

FEBS Letters

Wood, Paul M.¹  Gordon-Smith, Duncan¹  Higham, Christopher W.¹  Dempsey, Christopher E.¹ 

[1] Department of Biochemistry, University of Bristol, University Walk, Bristol, BS8 1TD, UK

关键词: Anomeric specificity;    Cellobionolactone;    d-Cellobiose;    Cellobiose dehydrogenase;    Lactone hydrolysis;    Phanerochaete chrysosporium;    CDH;    cellobiose dehydrogenase;   

DOI  :  10.1016/0014-5793(94)00847-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The α- and β-anomers of d-cellobiose were resolved by 1H NMR spectroscopy. Addition of cellobiose dehydrogenase purified from the white-rot P. chrysosporium led to selective conversion of β-d-cellobiose. The product was identical to cellobionolactone as synthesized from Ca-cellobionate. Overnight incubation of the product led to an altered NMR spectrum, which was also obtained by incubation of cellobionolactone. The new spectrum matched that for Ca-cellobionate. The instability of cellobionolactone explains the detection of cellobionic acid as product in earlier studies.

【 授权许可】

Unknown