FEBS Letters | |
Modulation of AP‐1 activity by nitric oxide (NO) in vitro: NO‐mediated modulation of AP‐1 | |
Oh, Esther1  Tsuda, Masaaki1  Tsuchiya, Tomofusa1  Sano, Kuniaki1  Tabuchi, Akiko1  | |
[1] Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Okayama 700, Japan | |
关键词: Nitric oxide (NO) AP-1; Redox regulation; S-nitrosylation; Sodium nitroprusside (SNP); Transcriptional factor; | |
DOI : 10.1016/0014-5793(94)00839-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To understand the role of nitric oxide (NO) in controlling the specific DNA-binding activities of transcriptional factors, we investigated the in vitro effect of the NO-donor sodium nitroprusside (SNP) on the AP-1 activity of cultured mouse cerebellar granule cells. A gel-mobility assay showed that SNP inhibited AP-1 activity in the presence, but not the absence of dithiothreitol (DTT). This DTT-dependent inhibition of AP-1 activity by SNP corresponded with the activation of the chemical reactivity of SNP with DTT, which can be monitored by the production of nitrite (NO− 2). In contrast, diamide, a typical sulfhydryl oxidizing agent, inhibited AP-1 activity in the absence of DTT and its inhibitory effect was reversed competitively by DTT. Studies using structurally or functionally related analogues of SNP demonstrated that S-nitrosylation of the AP-1 moiety mediated by some NO-carriers but not by free NO, which can be produced by the chemical reaction of SNP with DTT, was responsible for the inhibition of AP-1 activity, suggesting NO-mediated regulation of the AP-1 transcriptional factor.
【 授权许可】
Unknown
【 预 览 】
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