Role of protein kinase C in cholinergic stimulation of lacrimal gland protein secretion

FEBS Letters

Dartt, Darlene A.¹  Hodges, Robin R.¹  Dicker, Deanna M.¹  Zoukhri, Driss¹ 

[1] Schepens Eye Research Institute and Harvard Medical School, Boston, MA 02114, USA

关键词: Carbachol;    Exocrine gland;    Protein kinase C;    Phorbol ester;    Signal transduction;    Exocytosis;   

DOI  :  10.1016/0014-5793(94)00824-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The purpose of this study was to determine the role of protein kinase C (PKC) isozymes in carbachol-induced protein secretion in the lacrimal gland. Three isoforms of PKC are present in rat lacrimal gland: PKC-α, -δ and -g3. Carbachol translocated PKC-ε during 5 s incubation. Pretreatment with PdBu for 0 to 4 h down-regulated PKC-α by 31% at 20 min, PKC-ε by 36% at 2 h, and PKC-δ by 37% at 4 h. A 2 h phorbol ester treatment inhibited carbachol-induced secretion completely at 1 min and partially at 5, and 20 min, but did not alter the carbachol-induced increase in the intracellular [Ca²⁺]. We conclude that PKC-α and -g3, but not PKC-δ, are implicated in cholinergic agonist-induced protein secretion in rat lacrimal gland.

【 授权许可】

Unknown