Effect of lysophosphatidic acid on the preimplantation development of mouse embryos

FEBS Letters

Aono, Toshihiro²  Tokumura, Akira¹  Yamano, Shuji²  Ishikawa, Hiromi²  Murayama, Shinji²  Kobayashi, Tsuzuki² 

[1]Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima 770, Japan

[2]Department of Obstetrics and Gynecology, School of Medicine, 3-18-15, Kuramoto, Tokushima 770, Japan

关键词: Embryo;    Lysophosphatidic acid;    Pertussis toxin;    Mouse;    Basic CM;    Whittingham's T6 medium supplemented with 0.4% bovine serum albumin;    hCG;    human chorionic gonadotropin;    LPA;    lysophosphatidic acid;    PT;    pertussis toxin;   

DOI  :  10.1016/0014-5793(94)00815-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of lysophosphatidic acid (LPA) on the preimplantation development of mouse embryos was examined. The blastocyst rates from 2-cell and 4-cell stage embryos were significantly higher in the presence of LPA at the pronuclear stage. However, the blastocyst rate from 4-cell stage embryos was significantly lower when LPA was added to the medium at the 4-cell stage than when it was added at the pronuclear stage. Furthermore, pretreatment of pronuclear stage embryos with pertussis toxin suppressed LPA-induced embryo development, suggesting that it is probably mediated by a Gi-protein-linked receptor.

【 授权许可】

Unknown