Differential inhibition of eukaryotic DNA polymerases by halenaquinol sulfate, a p‐hydroquinone sulfate obtained from a marine sponge

FEBS Letters

Shoji, Mami³  Kitagawa, Isao⁴  Shioda, Masaki²  Kano, Kazukaka²  Ikegami, Susumu¹  Kobayashi, Motomasa⁴  Yoshida, Shonen³ 

[1] Department of Applied Biochemistry, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 724, Japan;Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan;Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-chyo, Showa-ku, Nagoya 466, Japan;Faculty of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565, Japan

关键词: Halenaquinol sulfate;    DNA polymerase α;    DNA polymerase ε;    DNA polymerase δ;    Inhibition;   

DOI  :  10.1016/0014-5793(94)00777-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Halenaquinol sulfate, a p-hydroquinone sulfate obtained from a marine sponge, inhibited the activity of eukaryotic DNA polymerases in varying degrees; the K _i values for DNA polymerases α, β, δ and ε were 1.3, 80, 17.5 and 2.0 νM, respectively, whereas it was less effective against E. coli DNA polymerase I. The inhibition occurred competitively with each of dATP and dTTP, but non-competitively with dCTP, dGTP and the template DNA. Thus, halenaquinol sulfate is demonstrated to be a potential inhibitor of DNA polymerases α and ε, and be a useful tool for analysing the DNTP binding sites of DNA polymerases.

【 授权许可】

Unknown