FEBS Letters | |
Binding of GM1 ganglioside to a synthetic peptide derived from the lysosomal sphingolipid activator protein saposin B | |
Lamontagne, Sonia1  Potier, Michel1  Champagne, Marie-Josée1  | |
[1] Service de Génétique Médicale, Hôpital Sainte-Justine, et Département de Pédiatrie et de Biochimie, Université de Montréal, Montréal, Qué., H3T 1C5, Canada | |
关键词: Saposin B; GM1 ganglioside; Lysosomal disease; | |
DOI : 10.1016/0014-5793(94)00717-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Saposin B is a lysosomal sphingolipid activator protein which activates GM1 ganglioside hydrolysis by lysosomal β-galactosidase. To identify the structural elements of saposin B implicated in sphingolipid binding, we studied a synthetic peptide corresponding to a predicted α-helix, sapB-18, spanning residues 52–69 of saposin B. The circular dichroism spectrum of sapB-18 at pH 4.4 was consistent with a 44% α-helix content. As shown by intrinsic Tyr fluorescence studies of sapB-18, this peptide binds the GM1 ganglioside with a K d of about 7 μM. Thus, we suggest that a putative amphipathic α-helix between residues 52 and 69 of saposin B plays a major role in the recognition and binding of GM1 ganglioside by saposin B.
【 授权许可】
Unknown
【 预 览 】
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