Antioxidant system in rat testicular cells

FEBS Letters

Jégou, Bernard¹  Fouchard, Marie-Hélène¹  Bauché, Françoise¹ 

[1] GERM, INSERM CJF 91-04, Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, Bretagne, France

关键词: Testicular cell;    Glutathione;    Antioxidant enzyme;    Reactive oxygen;    Rat;   

DOI  :  10.1016/0014-5793(94)00709-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activity of the enzymes involved in the antioxidant defence — superoxide dismutase (SOD), glutathione peroxidase (GPx), reductase (GR) S-transferase (GST) — as well as the glutathione (GSH) levels were measured in different rat testicular cell populations. A differential distribution of these components among testicular cell types was clearly observed. Sertoli and peritubular cells had elevated SOD and GSH-dependent enzyme activities associated with a high GSH content. Compared with the somatic cells, pachytene spermatocytes (PS) round spermatids (RS) presented a different antioxidant system characterized by higher SOD activity and GSH content associated with very low GSH-dependent enzyme activity. Spermatozoa exhibited the same enzymatic system as PS and RS but were devoid of GSH. Interstitial tissue displayed high GSH content, moderate SOD and GSH-related enzyme activity except for GPx which was very elevated. It is concluded that the different categories of testicular cells probably display a highly variable susceptibility to oxidative stress.

【 授权许可】

Unknown