Enzyme‐aided construction of medium‐sized alditols of complete O‐linked saccharides

FEBS Letters

Penttilä, Leena¹  Maaheimo, Hannu¹  Renkonen, Ossi¹ 

[1] Institute of Biotechnology and Department of Biochemistry, University of Helsinki, PO Box 45, FIN-00014 University of Helsinki, Finland

关键词: Endo-β-galactosidase;    O-Linked saccharide;    GlcNAcβ1—6Gal;    In vitro synthesis;    NMR;    HPAEC;    Gal;    d-galactose;    GlcNAc;    N-acetyl-d-glucosamine;    GalNAc;    N-acetyl-d-galactosamine;    NMR;    nuclear magnetic resonance spectroscopy;    WEFT;    water eliminated fourier transformation;    DQFCOSY;    double quantum filtered correlation spectroscopy;    DEPT;    distortionless enhancement by polarization transfer;    HPAEC;    high-pH anion-exchange chromatography;    PAD;    pulsed amperometric detection;   

DOI  :  10.1016/0014-5793(94)00638-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have constructed by enzyme-aided in vitro synthesis a hexasaccharide alditol Galβ1—4GlcNAcβ1—6Galβ1—4GlcNAcβ1—6(Galβ1—3)GalNAc-ol and shown that it resists the action of endo-β-galactosidase from Bacteroides fragilis under conditions where a related pentasaccharide alditol, GlcNAcβ1—3Galβ1—4GlcNAcβ1—6(Galβ1—3)GalNAc-ol, was completely cleaved. Together with earlier results from this laboratory, our present data imply that endo-β-galactosidase from B. fragilis, apparently, can be used to distinguish between GlcNAcβ1—6Gal and GlcNAcβ1—3Gal units within linear backbone sequences of all known types of oligo-(N-acetyllactosamino)glycans.

【 授权许可】

Unknown