Inhibition of cellular response to platelet‐derived growth factor by low M r phosphotyrosine protein phosphatase overexpression

FEBS Letters

Raugei, Giovanni¹  Degl'Innocenti, Donatella¹  Rigacci, Stefania¹  Ramponi, Giampietro¹  Berti, Andrea¹ 

[1] Dipartimento di Scienze Biochimiche, Università di Firenze, Viale Morgagni 50, 50134 Florence, Italy

关键词: Phosphotyrosine protein phosphatase;    PTPase;    Platelet-derived growth factor;    PTPase;    phosphotyrosine protein phosphatase;    PDGF platelet-derived growth factor;    FCS;    fetal calf serum;    PAGE;    polyacrylamide gel electrophoresis;    IGF-1;    insulin-like growth factor;    PAO phenylarsine oxide;   

DOI  :  10.1016/0014-5793(94)00620-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of low M _r phosphotyrosine protein phosphatase (PTPase) in the control of cell proliferation was studied. A synthetic gene coding for PTPase was transfected and expressed in NIH/3T3 fibroblasts. The effects of the enzyme were particularly evident when cells were stimulated by platelet-derived growth factor (PDGF). The mitogenic response to PDGF was decreased and the inhibition reached 90%. This effect was more pronounced with respect to fetal calf serum stimulation. Hormone-dependent autophosphorylation of the PDGF receptor was significantly reduced. These results demonstrate that low M _r PTPase, a cytosolic enzyme, not only affects cellular response to PDGF but also reduces the membrane receptor autophosphorylation.

【 授权许可】

Unknown