Molecular cloning of a bovine MSH receptor which is highly expressed in the testis

FEBS Letters

Höllt, Volker¹  Meyerhof, Wolfgang³  Vanetti, Mirko²  Schönrock, Christiane³ 

[1] Institut für Pharmakologie und Toxikologie, Otto von Guericke Universität, Leipzigerstraße 44, Magdeburg, Germany;Physiologisches Institut, Ludwig Maximilian Universität München, Munich, Germany;Institut für Zellbiochemie und klinische Neurobiologie, Universität Hamburg, Hamburg, Germany

关键词: G-protein coupled receptor;    α-MSH;    POMC;    Testis;    Adenylate cyclase;    PCR;    spermatogenesis;   

DOI  :  10.1016/0014-5793(94)00619-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel G protein-coupled receptor (BDF3) was isolated from bovine genomic DNA by a combined approach of polymerase chain reaction (PCR) and hybridization techniques. The predicted amino acid sequence is 317 amino acids in length and displays 80% homology to the human α-MSH receptor MC1. Stably transfected into CHO-K1 cells, BDF3 mediates an increase of intracellular cAMP-levels following incubation with NLE-α-MSH, a potent α-MSH analog. The stimulation with ACTH_1–10 is only moderate and γ-MSH is ineffective. Northern blot analysis of bovine tissues revealed that the BDF3 gene is higly expressed in the testis as a single 2.3 kb mRNA species, suggesting an involvement of the BDF3 receptor in spermatogenesis.

【 授权许可】

Unknown