Endothelin‐1 increases the levels of mRNA and protein of muscarinic acetylcholine receptors and c‐fos mRNA in cerebellar granule cells

FEBS Letters

Chuang, De-Maw¹  Fukamauchi, Fumihiko¹ 

[1] Section on Molecular Neurobiology, Biological Psychiatry Branch, National Institute of Mental Health, National Institutes of Health, Bldg. 10, Rm. 3N212, 9000 Rockville Pike, Bethesda, MD 20892, USA

关键词: Endothelin-1;    Muscarinic acetylcholine receptor mRNA;    c-fos mRNA;    Northern blot hybridization;    Immunoprecipitation;    Cerebellar granule cells;    ET-1;    endothelin-1;    mAChR;    muscarinic acetylcholine receptor;    PDBu;    phorbol dibutyrate;   

DOI  :  10.1016/0014-5793(94)00611-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endothelin-1 (ET-1) induced a time- and dose-dependent increase in the levels of mRNA of m₂- and m₃-muscarinic acetylcholine receptors (mAChRs) in cultured cerebellar granule cells. The levels of immunoprecipitable m₃-mAChR protein and total mAChR binding sites were also increased by ET-1 treatment. The up-regulation of m₂- and m₃-mAChR mRNA was blocked by phorbol ester pretreatment to inhibit ET-1-stimulated phosphoinositide hydrolysis and was preceded by an increase in c-fos mRNA levels. Treatments that prevented ET-1-induced c-fos mRNA increase also abolished the subsequent m₂-and m₃-mAChR mRNA up-regulation, suggesting that c-Fos protein is involved in the ET-1-induced mAChR expression.

【 授权许可】

Unknown