Effects of cobalt on haem proteins of erythropoietin‐producing HepG2 cells in multicellular spheroid culture

FEBS Letters

Holtermann, G.¹  Acker, H.¹  Görlach, A.¹  Fandrey, J.² 

[1]Max Planck Institut für Molekulare Physiologie, Rheinlanddamm 20, D-44139 Dortmund, Germany

[2]Physiologisches Institut I, Rheinische Friedrich Wilhelms Universität, Nuβallee 11, D-53115 Bonn, Germany

关键词: HepG2 cell;    Erythropoietin;    Haem protein: H2O2;    Cobalt;    Oxygen sensing;   

DOI  :  10.1016/0014-5793(94)00607-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hypoxia-induced increase of spectrophotometrically measured light absorption at 560 nm, considered as reduced cytochrome b, in HepG2 cells is diminished after exposure to cobalt chloride (50 or 100 μM) for 18–36h. The redox state of cytochrome c and cytochrome aa ₃, however, remains stable, indicating a particular affinity of cytochrome b for cobalt. Erythropoietin production of HepG2 cells increases after application of cobalt chloride, whereas H₂O₂ production, as measured by the dihydrorhodamine technique, decreases. It is concluded that cobalt stimulates a signal cascade with cytochrome b as receptor and H₂O₂ as second messenger for regulating erythropoietin production.

【 授权许可】

Unknown