Myelin membrane biogenesis by oligodendrocytes Developmental regulation of low molecular weight GTP‐binding proteins

FEBS Letters

Simons, Kai²  Huber, Lukas A.²  Pfeiffer, S.E.¹  Madison, Dana L.¹ 

[1] Department of Microbiology, School of Medicine, University of Connecticut, Farmington, CT 06030-3205, USA;Department of Cell Biology, European Molecular Biology Laboratory, Heidelberg, Germany

关键词: GTP-binding protein;    Rab protein;    Oligodendrocyte;    Myelin biogenesis;    Vesicular trafficking;    PDGF-BB;    B-subunit homodimer of platelet derived growth factor;    bFGF;    basic fibroblast growth factor;    GD3 +;    cells expressing the ganglioside GD3;    Pro-OL;    pro-oligodendroblasts;    GalC;    galactosylcerebroside;    OL;    oligodendrocytes;    MBP;    myelin basic protein;   

DOI  :  10.1016/0014-5793(94)00562-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oligodendrocytes synthesize dramatic amounts of myelin membrane. We hypothesized that this requires unique aspects of vesicular trafficking. Specific stages of the oligodendrocyte lineage were assayed for low molecular weight GTP-binding proteins implicated in the regulation of vesicular transport pathway (two dimensional gel electrophoresis, [α-³²P]GTP overlay). Consistent with the hypothesis, as oligodendrocytes differentiate from early progenitors to mature myelin-producing cells, ≥≥ 12 small GTP-binding proteins become up-regulated. Myelin membrane also has a complex pattern of GTP-binding proteins. Several of these proteins may be specific to oligodendrocytes, suggesting that oligodendrocytes may utilize cell-type specific GTP-binding proteins for biogenesis and maintenance of the myelin membrane.

【 授权许可】

Unknown