Distinguishing between duplex and hairpin forms of RNA by 15N–1H heteronuclear NMR

FEBS Letters

Pardi, Arthur¹  Nikonowicz, Edward P.¹  Aboul-ela, Fareed¹ 

[1] Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA

关键词: :Heteronuclear NMR;    RNA structure;    RNA hairpin;    RNA duplex;    15N;    Isotope;   

DOI  :  10.1016/0014-5793(94)00564-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A general method is described for distinguishing RNA hairpins from RNA duplexes by application of two-dimensional filtered nuclear Overhauser enhancement spectra on a 1:1 mixture of unlabeled and 99% ¹⁵N-labeled molecules. The method is applied to the RNA dodecamer rGGCGCUUGCGUC which can form an intramolecular hairpin under low salt conditions and a duplex in high salt. This procedure allows unambiguous identification of RNA hairpins or duplexes under the same conditions that are used in the NMR solution structure determination.

【 授权许可】

Unknown