FEBS Letters | |
NAD(P)H‐flavin oxidoreductase from the bioluminescent bacterium, Vibrio fischeri ATCC 7744, is a flavoprotein | |
Inouye, Satoshi1  | |
[1] Yokohama Research Center, Chisso Corp., Kanazawa-ku, Yokohama 236, Japan | |
关键词: Reduced flavin; Bacterial luciferase; NADH oxidase; Nitroreductase; Diaphorase; | |
DOI : 10.1016/0014-5793(94)00528-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The NAD(P)H-flavin oxidoreductase gene from the bioluminescent bacterium, Vibrio fischeri ATCC 7744, was expressed in Escherichia coli, and the enzyme purified using Cibacron Blue 3G-A affinity column chromatography from crude extracts in a single step. The purified enzyme had a typical flavoprotein absorption spectrum and flavin mononucleotide (FMN) was identified as a prosthetic group, non-covalently bound in a molar ratio of 1:1. The enzyme catalyzed the electron transfer from NADH via FMNH2 to various other electron acceptors. Reduced flavin produced by flavin reductase participated non-enzymatically in the following reactions: H2O2-forming NADH oxidase-like, oxygen-insenstive nitroreductase-like, diaphorase (quinone reductase)-like and bacterial luciferase reactions.
【 授权许可】
Unknown
【 预 览 】
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