Vitamin D status modulates rat colonic M3 muscarinic receptor characteristics and coupling to guanylate cyclase

FEBS Letters

Wali, Ramesh K.¹  Tien, Xiao-Ying¹  Sitrin, Michael D.¹  Bissonnette, Bruce M.¹  Brasitus, Thomas A.¹  Khare, Sharad¹  Wilson, David¹ 

[1] Department of Medicine, MC 4076, The University of Chicago, Hospitals and Clinics, 5841 S. Maryland Avenue, Chicago, IL 60637, USA

关键词: Signal transduction;    Cyclic GMP;    Polyphosphoinositide;    Cyclic nucleotide;    Rat large intestine;    Muscarinic agonist;   

DOI  :  10.1016/0014-5793(94)00498-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present studies were conducted to determine whether [³H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC₅₀ values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors (B _max) were significantly lower, and dissociation constants (K _d) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.

【 授权许可】

Unknown