期刊论文详细信息
FEBS Letters
Biosynthetic lipid‐tagging of antibodies
Keinänen, Kari1  Laukkanen, Marja-Leena1 
[1] VTT Biotechnology and Food Research, P.O. Box 1500, FIN-02044 VTT Espoo, Finland
关键词: Immunoliposome;    Antibody engineering;    E. coli lipoprotein;    Lipid modification;   
DOI  :  10.1016/0014-5793(94)00313-0
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
PDF
【 摘 要 】

Chemical conjugation of fatty acids to antibodies generates lipid-modified molecules which have found use in the targeting of liposome-mediated drug delivery and in liposome-based immunoassays. Alternatively, bacterial expression of antibodies as single-chain Fv fragments fused to lipoprotein signal peptide and N-terminal sequence leads to in vivo enzymatic addition of a single glycerolipid group at the N-terminus of the molecule. This lipid-modification converts the antibody from a soluble protein into a functional membrane-bound molecule. These biosynthetically lipid-tagged antibodies may prove useful for immobilization of antibodies to membranes in various biotechnological applications.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201912020299601ZK.pdf 413KB PDF download
  文献评价指标  
  下载次数:3次 浏览次数:15次