Biosynthetic lipid‐tagging of antibodies

FEBS Letters

Keinänen, Kari¹  Laukkanen, Marja-Leena¹ 

[1] VTT Biotechnology and Food Research, P.O. Box 1500, FIN-02044 VTT Espoo, Finland

关键词: Immunoliposome;    Antibody engineering;    E. coli lipoprotein;    Lipid modification;   

DOI  :  10.1016/0014-5793(94)00313-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chemical conjugation of fatty acids to antibodies generates lipid-modified molecules which have found use in the targeting of liposome-mediated drug delivery and in liposome-based immunoassays. Alternatively, bacterial expression of antibodies as single-chain Fv fragments fused to lipoprotein signal peptide and N-terminal sequence leads to in vivo enzymatic addition of a single glycerolipid group at the N-terminus of the molecule. This lipid-modification converts the antibody from a soluble protein into a functional membrane-bound molecule. These biosynthetically lipid-tagged antibodies may prove useful for immobilization of antibodies to membranes in various biotechnological applications.

【 授权许可】

Unknown