Synthetic peptides in the determination of hepatitis A virus T‐cell epitopes

FEBS Letters

Kozhich, Alexander T.¹  Kulik, Liudmila N.¹  Gabrielian, Andrei E.²  Tchikin, Leonid D.¹  Ivanov, Vadim S.¹  Ivanov, Vadim T.¹ 

[1] Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117871, Moscow, GSP-7, Russian Federation;Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117984 Moscow B-334, Russian Federation

关键词: Amphipathicity prediction;    T-cell proliferation;    Immunodominant promiscuous site;   

DOI  :  10.1016/0014-5793(94)00427-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Computer search for probable T-epitopes of hepatitis A virus capsid proteins was performed using an integrated set of programs. Eight segments of the VP1, VP2, VP3 and VP4 proteins were chosen and synthesised. Five peptides previously examined as probable B-epitopes were used as well. All the peptides were tested for their ability to stimulate proliferation of lymph node T-cells primed with synthetic peptides. Almost all predicted T-epitopes affected the T-cell proliferation. None of the peptides had mitogenic activity. We demonstrated that regions 17–33 and 276–298 of VP1 are possible immunodominant promiscuous sites activating lymphocytes of all mouse haplotypes.

【 授权许可】

Unknown