Expression and functional characterisation of a synthetic version of the human D4 dopamine receptor in a stable human cell line

FEBS Letters

Coldwell, Martin C.²  Winn, Fiona M.²  Robinson, Jeff H.¹  Cook, Ted¹  Herrity, Nicole¹  Ball, Stuart¹  Gloger, Israel S.¹  Boyfield, Izzy²  McHale, Mark¹ 

[1] Department of Biotechnology, SmithKline Beecham Pharmaceuticals, Coldharbour Road, Harlow, Essex, CM19 5AD, UK;Department of Psychiatry, SmithKline Beecham Pharmaceuticals, Coldharbour Road, Harlow, Essex, CM19 5AD, UK

关键词: D4 dopamine receptor;    Synthetic;    Stable expression;    Functional characterisation;    HEK 293 cell;   

DOI  :  10.1016/0014-5793(94)00423-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A synthetic version of the human D₄ (hD4) dopamine receptor was prepared. The G/C content of the natural gene was reduced by 14% without altering the amino acid composition of the corresponding protein sequence. HEK293 cells were transfected with the synthetic hD4 gene and stable clones resistant to G418 selected. The hD4 receptor expressed from the synthetic gene had identical pharmacological characteristics to the native hD4 receptor [(1991) Nature 350, 610–619; (1992) Nature 358, 149–152]. Functional studies with cells expressing the synthetic hD4 gene indicated negative coupling of this receptor to adenylate cyclase.

【 授权许可】

Unknown