The covalent maleimidobenzoyl‐actin‐myosin head complex

FEBS Letters

Bertrand, Raoul¹  Kassab, Rhida¹  Derancourt, Jean¹ 

[1] Centre de Recherches de Biochimie Macromoléculaire du CNRS, INSERM U 249, Université de Montpellier I, Route de Mende, BP 5051, 34033 Montpellier Cedex, France

关键词: F-actin;    G-actin;    Actomyosin interaction;    Actin-myosin head cross-linking;    Actin subdomain-2;   

DOI  :  10.1016/0014-5793(94)00398-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified the region of actin involved in the covalent coupling of maleimidobenzoyl-G-actin to the central 50 kDa segment of the myosin-S-1 heavy chain by analyzing the structure of the maleimidobenzoyl-G-actin-S-1 conjugate using selective proteolytic digestions, amino acid sequence determinations and novel cross-linking reactions between S-1 and different maleimidobenzoyl-G-actin derivatives. The cross-linking is shown to occur only on the stretch of residues 48–67 in actin subdomain-2 with Lys-50, residing on the outer part of the DNase-I-binding loop, as the most likely site of cross-linking. Because the maleimidobenzoyl-F-actin-S-1 complex undergoes the same coupling process, the data provide experimental evidence in favor of the recent model of the rigor F-actin-S-1 complex suggesting a close contact between structural elements of the lower domain of the 50 kDa fragment and the top of actin subdomain-2.

【 授权许可】

Unknown