Site‐specific incorporation of photofunctional nonnatural amino acids into a polypeptide through in vitro protein biosynthesis

FEBS Letters

Sisido, Masahiko¹  Takai, Kazuyuki²  Yokoyama, Shigeyuki²  Hohsaka, Takahiro¹  Sato, Ken¹ 

[1] Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 227, Japan;Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Nonnatural amino acid;    Photofunction;    Protein biosynthesis;    Minor codon;    Phenylazophenylalanine;    Anthrylalanine;   

DOI  :  10.1016/0014-5793(94)00381-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nonnatural amino acids with photofunctional groups were incorporated site-specifically into a polypeptide by using in vitro protein synthesizing system. The nonnatural amino acids were attached to tRNA_CCU through chemical misacylation method, and added to the in vitro system with a mRNA containing a single AGG codon. l-p-Phenylazophenylalanine, l-2-anthrylalanine, l-1-naphthylalanine, l-2-naphthylalanine and l-p-biphenylalanine were successfully incorporated into a polypeptide, but l-1-pyrenylalanine was not. The polypeptides containing the nonnatural amino acids showed photofunctionalities.

【 授权许可】

Unknown