Molecular cloning of the canine angiotensin II receptor

FEBS Letters

Bradley, John²  Robertson, Mark J.²  Clark, Adrian J.L.¹  Burns, Louise¹  Clark, Kenneth L.² 

[1] Department of Chemical Endocrinology, St Bartholomew's Hospital Medical College, London, EC1A 7BE, UK;Department of Cardiovascular and Respiratory Pharmacology, Glaxo Group Research, Park Road, Ware, Hertfordshire, SG12 0DP, UK

关键词: Angiotensin receptor;    Non-peptide antagonists;    cDNA cloning;    Dog aorta;    Vascular contractility;    COS cell;   

DOI  :  10.1016/0014-5793(94)80307-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Canine aortic strip studies revealed insensitivity of angiotensin II (AII)-induced aortic contraction to inhibition by the non-peptide antagonist DuP753 (pK _B = 6.7 ± 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT₁ AII receptor. Expression of this cDNA in COS-7 cells indicated a low affinity of DuP753 for the cloned receptor (K _D = 92 nM). The predicted amino acid sequence is highly homologous to other mammalian AT₁ receptors; sequence comparisons suggest the pharmacological difference may be the result of a threonine residue in position 163 in the IVth transmembrane domain.

【 授权许可】

Unknown