Collagen‐binding recombinant fibronectin fragments containing type II domains

FEBS Letters

Etzerodt, Michael¹  Thøgersen, Hans C.¹  Skorstengaard, Karua¹  Holtet, Thor L.¹ 

[1] Laboratory of Gene Expression, Department of Chemistry, University of Aarhus, Gustav Wieds Vej 10, DK-8000 Aarhus, Denmark

关键词: Fibronectin;    Type II domains;    Gelatin-binding;    Refolding;    Factor Xa;    FN;    fibronectin;    GBF;    gelatin-binding fragment;    HPLC;    high-performance liquid chromatography;    FX;    plasma factor X;    PMSF;    phenylmethyl sulfonyl flouride;    NTA;    nitrilo-triacetic acid;   

DOI  :  10.1016/0014-5793(94)80604-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Each of the two type II domains and four larger fragments, containing one or two type II domains of fibronectin, have been expressed in Escherichia coli. A special vector, containing a fragment encoding the cleavage site for Factor X_a, Ile-Glu-Gly-Arg, inserted immediately before the protein fragment of interest, was used. After treatment of the purified fusion proteins with reduced/oxidized glutathione, the correctly folded fibronectin fragments were released by proteolytic digestion with Factor X_a. The largest fragment, consisting of two type II and two type I domains, was the only fragment able to bind to immobilized gelatin.

【 授权许可】

Unknown