Transcription factor GATA‐1‐multiprotein complexes and chicken erythroid development

FEBS Letters

Davie, James R.¹  Penner, C.Gail¹ 

[1] Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manitoba, 770 Bannatyne Ave., Winnipeg, Man., R3E OW3, Canada

关键词: GATA-1;    Transcription factor;    Hemoglobin switching;    Chicken erythropoiesis;   

DOI  :  10.1016/0014-5793(94)80515-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chicken erythrocyte transcription factor, GATA-1, is associated with several non-DNA binding proteins. We show that GATA-1 multiprotein complexes exist in primitive and definitive erythrocytes. These complexes bind to GATA motifs of the p-globin promoter and histone H5 enhancer with high affinity, and to the chicken β-globin promoter specialized TATA element and enhancer GATA with low affinity. The low affinity β-globin TATA element would allow basal transcription factors to displace the GATA-1 multiprotein complex. Further, our results suggest that p-globin promoter's low affinity Sp1 binding site and reduced levels of Sp1 in definitive cells prevent its expression in these cells.

【 授权许可】

Unknown