Cloning and expression of the bovine sodium‐ and chloride‐dependent noradrenaline transporter

FEBS Letters

Bönisch, Heinz¹  Lingen, Bettina¹  Brüss, Michael¹ 

[1] Institut für Pharmakologie und Toxikologie, Universität Bonn, Reuterstr. 2b, D-53113 Bonn, Germany

关键词: Noradrenaline transporter;    Plasma membrane;    Adrenal medulla;    Protein kinase C;   

DOI  :  10.1016/0014-5793(94)80508-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding a functional bovine, tricyclic antidepressant-sensitive noradrenaline transporter has been identified by screening a λgt11 cDNA library of the bovine adrenal medulla using the cDNA of the human noradrenaline transporter [1991, Nature 350, 350-354]. The sequence predicts a protein of 615 amino acids (M _r, 68 900). The bovine transporter shares 93% amino acid identity with the human sequence, but displays two more consensus sites for phosphorylation by protein kinase C. Transient expression of the transporter in COS-7 cells resulted in a sodium- and chloride-dependent uptake of noradrenaline with a pharmacology typical for a neuronal noradrenaline transporter.

【 授权许可】

Unknown